Protocols

  1. SDS-PAGE
  2. Western Blot
  3. Faellung von Proteinen
  4. IF Prep cells tissues
  5. RNA isol large scale
  6. Agarose gel extraction
  7. RNA-Isolierung
  8. Site-directed Mutagenesis
  9. Competent bacteria and transformation
  10. Long Range PCR TripleMaster System
  11. Partial trypsin digestion of cornified envelopes
  12. Myko-PCR
  13. Immunfluoreszenz
  14. Isolation murine keratinocytes
  15. Plasmid preparation
  16. Culture of HM1-cells with feeders
  17. Culture of HM1-cells
  18. Chromosome FISH
  19. Superovulation matings BCI
  20. Southern Blotting
  21. Ca-phosph transfection
  22. Nucleofection MDCK II
  23. Molecular cloning
  24. Cryo In situ hybridization
  25. Immunprecipitation
  26. Tissue fixation and embedding
  27. Whole mount in situ hybridization
  28. Transfektion von Säugerzellen
  29. Culture of HM1-cells with FCS
  30. Mirus keratinocyte transfection
  31. Herstellung von Mowiol
  32. Antigen Retrieval
  33. Kultivierung von Saeugerzellen
  34. Behandlung von Zellen mit Ciprofloxacin
  35. M2 Medium
  36. Mouse House Protocols
  37. PCR
  38. Dialyse
  39. cDNA-Synthese SuperScript
  40. Isolierung genomischer DNA aus Zellen und Geweben high quality
  41. Isolierung genomischer DNA fuer PCR
  42. Proteinbestimmung
  43. Northern Blotting
  44. RNA Isolation
  45. LIF production
  46. Purification of phage DNA from liquid cultures
  47. Two-dimensional gel electrophoresis
  48. Creating dA overhangs with Taq
  49. REZEPT für Ivomectin
  50. Silan coating of slides
  51. Hybrid selection assay
  52. Whole mount immunocytochemistry
  53. ZnSo4 stain of SDS PAGE gels
  54. Proteinexpression
  55. Total protein extracts prep
  56. Ammonsulfatfaellung
  57. HiPerFect Transfection Reagent Handbook, siRNA Transfection
  58. Epid Sheet Prep
  59. Cornified envelope prep
  60. Bradford
  61. ProLongGold Handhabung Invitrogen
  62. Vorbereitung von Deckglaeschen
  63. Primer loesen
  64. DNA Faellung fuer Transfektionsexperimente
  65. yellow blue tips
  66. Aliquoting ultra-pure water
  67. Buffers pH Meter
  68. dNTPs
  69. Cell proliferation assay Cell Signaling
  70. Culture mouse keratinocytes
  71. Fixation FISH
  72. GFP-Trap, Synchronisation HeLa HEK293
  73. Transfection Keratinocytes Lipofectamin
  74. mouse primary keratinocytes
  75. Poly L Lysin Beschichtung
  76. GFP-Pulldown Protokoll
  77. E coli DH5alpha NEB
  78. 3T3-J2 Feeder culture
  79. Zelllysate Keratinocyten
  80. RNA qPCR Standardprotokoll
  81. siRNA reverse trsf keratinoc
  82. XFect protocol
  83. Immunhistochemie Faerbung
  84. Whole mount Immunohistochem embroys
  85. Hoefer 2D Quick Start Guide
  86. Sample preparation for Colony PCR